ComputerOXYGEN AND AEROBIC METABOLISM
22
INSTRUCTIONS: Read the lab, analyze the data, and answer the questions.
INTRODUCTION
Oxygen plays a key role in aerobic cellular metabolism, facilitating the conversion of glucose,
protein, and lipids into usable energy. For every 6 molecules of oxygen used in the breakdown of
glucose, 6 molecules of carbon dioxide are produced, along with water and adenosine
triphosphate (ATP), according to the following equation:
C6H12O6 + 6O2 → 6CO2 + 6 H2O + energy (heat or ATP)
The average person uses 200–250 mL of O2 per minute at rest. This may increase to 2–3 L per
minute during heavy exercise and to twice that amount in highly trained athletes. The increase of
oxygen consumption is proportional to the amount of work performed up to a maximum level
which is dependent on conditioning. At the start of exercise, anaerobic metabolism is used
briefly, but this quickly changes to aerobic metabolism as blood flow to muscles increases.
As O2 is consumed and CO2 is produced by muscle cells (and other cells), a pressure gradient is
created between the cells, the interstitial fluid, and the bloodstream. A marked lowering of O2 in
interstitial fluid as it is used up by cells leads to O2 diffusion from the bloodstream. While
dissolved O2 provides an immediate supply to replenish the interstitial fluid and cells, the
majority of O2 is carried on hemoglobin molecules. Oxyhemoglobin dissociates more readily as
the oxygen concentration is lowered (and CO2 concentration increased), rapidly replenishing the
supply of dissolved O2. CO2 diffuses from active cells (where it is produced in high
concentration) to the interstitial fluid and bloodstream, where it is transported to the lungs mainly
as bicarbonate. In the lungs, the opposite is true. O2 follows a pressure gradient from the alveoli
into the bloodstream, and CO2 from the bloodstream into the alveoli.
In this experiment, you will measure tidal volumes and oxygen concentrations of deeply inhaled
and exhaled air at rest and after exercise. You will use these measurements to calculate the
resulting differences in oxygen consumption.
Important: Do not attempt this experiment if you have pulmonary or musculoskeletal problems
that might be aggravated by exercise.
OBJECTIVES
In this experiment, you will
•
Obtain graphical representation of tidal volume and change in O2 concentration with
breathing at rest and after exercise.
• Calculate oxygen consumption at rest and after exercise.
• Correlate your findings with clinical situations.
Human Physiology with Vernier
22 – 1
Computer 22
MATERIALS
computer
Vernier computer interface
Logger Pro
Vernier Spirometer
Vernier O2 Gas Sensor
Vernier O2-Spirometer adapter
disposable bacterial filter
nose clip
PROCEDURE
Part I Oxygen utilization at rest
1. Assemble the apparatus (see Figure 1).
a. Insert the O2 Gas Sensor into the vertical
opening of the O2-Spirometer adapter.
b. Identify the side of the Spirometer head
marked “Inlet” and insert it into the adapter
(the larger diameter side).
c. Attach the bacterial filter to the smaller end
of the adapter as shown. Caution: Do not
puncture the filter material inside the
bacterial filter.
Figure 1
2. Connect the Spirometer and the O2 Gas Sensor to the Vernier computer interface. Open the
file “22 Aerobic Metabolism” from the Human Physiology with Vernier folder. Hold the
apparatus in one or both hands. Brace your arm(s) against a solid surface, such as a table, and
click
. Check Spirometer in the pop-up box that appears. Do not zero the O2 Gas
Sensor. Note: The Spirometer must be held straight up and down during data collection (see
Figure 1).
3. Collect inhalation and exhalation data.
a. Put on the nose clip.
b. Click
to begin data collection.
c. Taking deep breaths, begin data collection with an
inhalation and continue to breathe in and out. Each
respiratory cycle should last 8–10 s (4–5 s per
inhalation and 4–5 s per exhalation). Continue
breathing in this fashion for the duration of the
experiment. Data collection will continue for 40 s.
4. Click the Next Page button, , to see the volume data.
If the baseline on the top graph has drifted, use the
Baseline Adjustment feature to bring the baseline
volumes closer to zero, as in Figure 2.
22 – 2
Figure 2
Human Physiology with Vernier
Oxygen and Aerobic Metabolism
5. Place the cursor on the second peak of the volume graph. Click and drag down to the valley
that follows it. Enter the y value displayed in the lower left corner of the graph to the
nearest 0.1 L as Breath 1 Pre-exercise Tidal Volume in Table 1. Repeat for a total of three
breaths, entering the y values for each breath in Table 1. Place the cursor on the second
peak of the oxygen gas vs. time graph. Click and drag down to the valley that follows it.
Enter the y value displayed in the lower left corner of the graph to the nearest 0.01 % as
Pre-exercise ∆O2 Concentration in Table 1. Repeat for a total of three breaths, entering the y
values for each breath in Table 1.
Part II Oxygen utilization during exercise
10. Clear your data by choosing Clear All Data from the Data menu. Return to Page 1.
11. Begin running in place for 2 minutes, moving arms as well as legs.
12. At the end of two minutes, pick up the apparatus in one or both hands. Brace your arm(s)
against a solid surface, such as a table, and click
. Check Spirometer in the pop-up box
that appears. Do not zero the O2 Gas Sensor. Note: The Spirometer must be held straight up
and down during data collection (see Figure 1).
13. Collect inhalation and exhalation data.
a. Put on the nose clip.
b. Click
to begin data collection.
c. Taking deep breaths, begin data collection with an inhalation and continue to breathe in
and out. Each respiratory cycle should last 8–10 s (4–5 s per inhalation and 4–5 s per
exhalation). Continue breathing in this fashion for the duration of the experiment. Data
collection will continue for 40 s.
14. Click the Next Page button, , to see the volume data. If the baseline on the top graph has
drifted, use the Baseline Adjustment feature to bring the baseline volumes closer to zero, as
in Figure 2.
15. Place the cursor on the second peak of the volume vs. time graph. Click and drag down to the
valley that follows it. Enter the y value displayed in the lower left corner of the graph to the
nearest 0.1 L as Breath 1 Post-exercise Tidal Volume in Table 1.
16. Repeat Step 15 for a total of three breaths, entering the y values for each breath in Table 1.
17. Place the cursor on the second peak of the Oxygen Gas vs. Time graph. Click and drag down
to the valley that follows it. Enter the y value displayed in the lower left corner of the graph
to the nearest 0.01% as Post-exercise ∆O2 Concentration in Table 1.
18. Repeat Step 17 for a total of three breaths, entering the y values for each breath in Table 1.
19. Compute the average value for each of the parameters measured and enter those values in
Table 1.
Human Physiology with Vernier
22 – 3
Computer 22
DATA
Flow rate, volume, and percent oxygen gas graphs collected while breathing at rest.
Table 1
∆O2 Concentration (%)
Tidal volume (L)
22 – 4
Breath
Pre-exercise
Post-exercise
Pre-exercise
Post-exercise
1
4.20
4.20
1.11
2.40
2
4.00
4.30
1.11
2.58
3
4.50
4.60
1.33
2.40
4
4.20
4.40
1.18
2.46
Average
4.23
4.38
1.18
2.46
Human Physiology with Vernier
Oxygen and Aerobic Metabolism
DATA ANALYSIS QUESTIONS
1. Compare the tidal volumes of the four breaths analyzed both at rest and post-exercise. Is it
important for this experiment that resting and post-exercise tidal volumes be similar?
2. Inhaled oxygen should have the same concentration at rest and post-exercise (approximately
21%), yet the peak oxygen concentration values in the post-exercise run never achieve this
value. What aspect of the experimental design accounts for this finding?
3. Use the average values for Tidal Volume and ∆O2 Concentration (%) from Table 1 to
calculate the average O2 consumed pre- and post-exercise per breath and over the combined
four breaths. Show your calculations.
Pre-Exercise:
∆O2 Concentration (%) Tidal Volume (L) = O2 consumed per breath
O2 consumed per breath 4 breaths = O2 consumed over that time interval
Post-Exercise:
∆O2 Concentration (%) Tidal Volume (L) = O2 consumed per breath
O2 consumed per breath 4 breaths = O2 consumed over that time interval
4. CO2 was not measured. What would you expect the volume of exhaled CO2 to be in this
experiment at rest and after exercise?
Human Physiology with Vernier
22 – 5
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