Pepsin Lab – data analysisWe are examining the digestion of proteins in egg white
(mostly Albumin) by the enzyme pepsin, over 5 days, in 6
different pH levels.
• What is the biggest question of our experiment?
• Do you have a hypothesis?
The Data
Digestion of egg white in a glass tube, measures in mm.
pH 1
day #
0
1
2
3
4
5
sample I (mm)
0
2.7
5.3
8
11.2
15.5
II
The Data
Digestion of egg white, in mm, at pH 1
I
day #
I
II
I-X
II – X
0
0
0.5
0.1
2.5
1
2.7
1
3.5
5.5
2
5.3
3
5.9
7.2
3
8
3.2
8.5
11.2
4
11.2
4
12.1
15.9
5
15.5
4.5
16.5
17.5
Digestion in mm, pH 1
day #
0
1
2
3
4
5
I
0
2.7
5.3
8
11.2
15.5
II
0.5
1
3
3.2
4
4.5
I-X
0.1
3.5
5.9
8.5
12.1
16.5
II – X
2.5
5.5
7.2
11.2
15.9
17.5
Digestion in mm, pH 2
day #
0
1
2
3
4
5
I
0.5
4
8
13
16
16.5
II
0.3
2.7
7.9
11
16
15.4
I-X
2
2.9
4.2
4.2
5.3
5.3
II – X
2.5
7.5
12.2
15.2
19
20.1
What are
the
variables in
our
experiment?
– Time (days)
– pH (0-5)
– Digestion (mm)
Digestion of egg white, in mm. pH 1
day #
0
1
2
3
4
5
sample I (mm)
0
2.7
5.3
8
11.2
15.5
What is the best
way to represent
this data in a
graph?
Digestion of egg white, in mm. pH 1
day #
0
1
2
3
4
5
sample I (mm)
0
2.7
5.3
8
11.2
15.5
x-axis, independent variable (or the cause)
y-axis, dependent variable (or the effect)
Digestion in mm, pH 1
day #
0
1
2
3
4
5
I
0
2.7
5.3
8
11.2
15.5
II
0.5
1
3
3.2
4
4.5
I-X
0.1
3.5
5.9
8.5
12.1
16.5
II – X
2.5
5.5
7.2
11.2
15.9
17.5
Digestion in mm, pH 2
day #
I
II
I-X
II – X
0
0.5
5
10
15
20
20.5
0.3
4.7
9.5
16
19.5
20
1.5
2.9
4.2
4.2
5.3
5.3
3
7.5
12.2
18.5
23
20.1
1
2
3
4
5
What is the best way to represent data
from different pH conditions in one graph?
x-axis, independent variable (or the cause)
y-axis, dependent variable (or the effect)
The slope of the linear line is the rate of the reaction.
What is the ideal pH for Pepsin’s activity?
Fitting a linear line and finding the slope,
for each sample separately.
What is the ideal pH for Pepsin’s activity?
Please draw each data set separately, so
we can clearly see the graph and data
points
What if the digestion of Pepsin is not linear?
Fit a non-linear line, and find the slope of the
tangent to the line.
Pepsin Lab Data
Trial 1 = I
Trial 2 = II
Trial 3 = O, I
Trial 4 = O, II
pH 0
Amount of digestion (mm)
Day 0
Day 1
Day 2
Day 3
Day 4
Trial
1
2.0
2.2
2.5
5.0
9.5
16.0
Trial
2
0.2
1.0
1.8
5.0
6.5
8.0
Trial
3
2.0
(bubbles)
2.1
3.8
8.1
11.2
20.5
Trial
4
1.5
2.1
4.0
10.8
18.3
26.0
pH1: 1.5, 2.0, 1.0, 1.5
pH 1
Amount of digestion (mm)
Day 0
Day 1
Day 2
Day 3
Day 4
Trial
1
1.3
1.5
4.0
7.9
10.0
16.0
Trial
2
1.5
2.0
5.5
8.5
12.0
17.0
Trial
3
0.5
1.0
5.1
6.0
8.5
15.5
Trial
4
1.0
1.5
5.6
7.3
10.6
16.5
pH 2
Amount of digestion (mm)
Day 0
Day 1
Day 2
Day 3
Day 4
Trial
1
2.0 (low
end)
2.3
11.5
21.4
25.9
empty
Trial
0.3
1.5
2.4
9.2
20.0
empty
2
Trial
3
1.0
1.0
1.0
1.0
2.0
1.8
Trial
4
2.0
2.2
3.0
3.0
3.0
2.5
Day 2
Day 3
Day 4
Trial (new): 0.5, 0,3,
trial O: 0.1, 0.0/0.1
pH 3
Amount of digestion (mm)
Day 0
Day 1
Trial
1
(new)
0.0
0.1
0.5
0.5
0.5
Trial
2
(new)
0.3
0.3
0.3
0.3
2.0
Trial
3X-I
2.0
2.1
2.2
2.2
2.2
4.0
Trial
4XII
0.5
0.7
2.0
2.0
3.0
7.7
Trial
5
O-I
(new)
0.1
0.1
0.1
0.1
0.5
Trial
6
O – II
(new)
0.0/0.1
0.0/0.1
0.1
0.1
0.5
pH 4
Trial
1
Amount of digestion (mm)
Day 0
Day 1
Day 2
Day 3
Day 4
1.5
1.6
1.8
2.2
3.0
5.0
Trial
2
2.5
2.5
3.5
3.5
3.5
5.2
Trial
3
1.0
1.5
2.0
3.0
7.4
10.4
Trial
4
2.0
2.0
2.5
5.8
11.0
12.9
pH 5
Amount of digestion (mm)
Day 0
Day 1
Day 2
Day 3
Day 4
Trial
1
1.2
1.3
2.0
2.0
2.0
3.0
Trial
2
1.8
1.8
1.8
1.8
2.0
3.3
Trial
3
1.1
1.9
2.3
2.5
2.5
3.5
Trial
4
1.0
1.2
1.2
1.5
1.5
2.7
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