Exploring Photosynthesis and Plant PigmentsStudent Name
4/12/2022
Instructions:
1. Please read all of the introduction and background information within the
investigative manual.
a. Once you have done so answer the prelab question BEFORE
completing any of the lab’s activities.
2. Once you have completed the prelab questions proceed to the activities
of the lab within the investigative manual.
a. As you read through the instructions for completing each activity
make sure you also:
i. Complete any instructions (append photos, etc)/ and answer
any questions found in the post lab questions for each
activity.
ii. Take the photos of your experiments in each activity as
directed below. IMPORTANT: Don’t clean-up your lab until you
know what portion of the experiment you need to take a
picture of.
3. Here is a video that will introduce you to the lab and its main concepts.
The student is encouraged to watch it.
a. Exploring Photosynthesis and Plant Pigments
Prelab Questions
1. A typical chloroplast within a plant contains several pigments. Which are
the most common pigments and what color of light will they absorb?
Which color will they reflect?
Chlorophyll a and b, and carotenoids are the most common pigments
found in plants. The pigments absorb blue and red light, while reflecting all
the other colors (purple, yellow, green, and orange).
2. In consideration of the 1st question, why do tree leaves change color in
the autumn?
Every leaf’s chlorophyll loses light to produce pigmentation, and the colors
that are absorbed stay in the leaves. Green and purple pigments are
broken down by chlorophyll.
1
© 2016 Carolina Biological Supply Company
3. What two molecules are made in the light-dependent reactions of
photosynthesis? Are these “energy-containing” molecules? Where did
they get their energy from and how are these used in the Calvin cycle?
ATP and NADPH are the molecules that are made. ADP and NADP+ are
responsible for generating the energy in these molecules. These two
molecules convert carbon dioxide into sugar.
4. As stated in the investigative manual, in this lab we will be investigating
the effect of both light and concentration of CO2 on the rate of
photosynthesis. BEFORE you begin activity #2 please read through the
activity and fill out the purpose and hypothesis statements found below
under the activity #2 heading.
Activity 1
Instructions:
1. Open the investigative manual. Locate all the needed materials
supplied in the kit and those you will need to supply yourself.
2. Lay them out in your work area.
3. Read through the entire set of instructions found in the investigative
manual for the activity to avoid making mistakes when you go to
execute the experiment.
4. Once you have read through the instructions go back to step 1 and
begin executing the experiment.
5. Please answer the questions below and/or append appropriate
representations of data (photos, graphs, etc). REMEMBER don’t clean
up until you have taken the appropriate photos of your experiment as
described below.
Photo 1 – Activity 1
Take a picture and insert the image(s) of your chromatography paper from step
12. of the “Procedure” section in activity 1 of the investigative manual:
2
© 2016 Carolina Biological Supply Company
Data Table 1: Chromatography of Plant Pigments
Band #
Distance from origin (mm)
Band Color/ Identification
1
1.5
Light yellow
2
2.5
Clear
3
4.5
Green
4
5
Light yellow
Solvent
front (from
Step 14.)
5
1. Many things can affect the evaporation of solvents during a
chromatography experiment. To allow researchers to compare results across
experiments under different conditions, scientists use an Rf value, that is the
ratio of the distance the pigment travelled to the distance the solvent
travelled as shown in the equation below:
Rf = (distance band traveled/distance solvent from traveled)
This number remains constant across trials. Calculate the Rf value for each
pigment.
1. 0.3cm
2. 0.5cm
3. 0.9cm
4. 1cm
3
© 2016 Carolina Biological Supply Company
2. What do the calculated Rf values indicate about the pigment within that
particular band? In other words, did that pigment have more or less
interaction (compared to the other pigments) with the chromatography
paper during the experiment?
4
© 2016 Carolina Biological Supply Company
Activity 2
Instructions:
1. Open the investigative manual. Locate all the needed materials
supplied in the kit and those you will need to supply yourself.
2. Lay them out in your work area.
3. Read through the entire set of instructions found in the investigative
manual for the activity to avoid making mistakes when you go to
execute the experiment.
4. Once you have read through the instructions go back to step 1 and
begin executing the experiment. REMEMBER don’t clean up until you
have taken the appropriate photos of your experiment as described
below.
Purpose statement: (This should be the question the experiment is attempting to
address. It should be written as a question.)
When leaves are under different light conditions or CO2 concentrations, will their
rate of photosynthesis increase?
Hypothesis statement: (This should be an “if/then” testable prediction that
addresses the question/purpose of the lab.)
In conditions where the light level is high and the CO2 level is low, then there will
be more photosynthetic activity.
Evidence/Claim statement: (This should be a statement regarding whether your
hypothesis was supported or refuted and what data/evidence allows you to
make this claim.)
I have been proven right with my hypothesis. The experiment showed that more
disks floated when put into the light than when put into the dark.
Reflection statement: (This should be a statement of what you learned, how your
understanding changed, if you have new questions, and what connections can
you make between the lab and the content in the book and other
assignments.)
5
© 2016 Carolina Biological Supply Company
Before doing this experiment, I had no idea how different the settings of light
and air made a difference in photosynthesis. Because baking soda produces
carbon dioxide, it made it hard for the disks to float.
Photo 1 – Activity 1
Take a picture and insert the image(s) of your 0.12% syringe from step 16. of the
“Light Experiment” section in activity 2 of the investigative manual:
Data Table 2: Leaf Disk Assay, Light Treatment
Time
(minutes)
A
(0.24%)
B
(0.24%)
Average of
A and B
A
(0.12%)
B
(0.12%)
0
5
10
15
20
25
30
35
40
45
50
55
60
6
© 2016 Carolina Biological Supply Company
Average of
A and B
Data Table 3: Leaf Disk Assay, Dark Treatment
Time
(minutes)
A
(0.24%)
B
(0.24%)
Average of
A and B
A
(0.12%)
B
(0.12%)
Average of
A and B
0
5
10
15
20
25
30
35
40
45
50
55
60
Graph 1 – Activity 1
Make a single line graph of your results from both the light and dark
experiments. Your y-axis should be the average number of disks floating from A
and B (column four from your tables) and the x-axis should be time in minutes.
Insert your graph below:
7
© 2016 Carolina Biological Supply Company
1. Having now completed activity 2 and your tables go back and fill in your
Evidence/Claim and Reflection statements and then answer the question(s)
below.
2. For both the dark and the light trials identify the independent, the
dependent, and the control variables.
Independent Variable: is light and dark
Dependent variable: is the disk of the leaf
Control variable: is the amount of time in minutes
3. In this activity how do floating disks correspond to the rate of photosynthesis?
4. How did the baking soda solution affect photosynthetic rates? If you were to
conduct an experiment with a 0% baking soda solution would a
photosynthetic rate be observed? Why or why not?
5. Proceed now to the next assignment in D2L entitled “Lab Kit: Exploring
Photosynthesis and Plant Pigments Formal Report”. Instructions for writing the
formal report are found in D2L. Please submit the formal report as a separate
assignment from this one within the appropriate submission folder.
8
© 2016 Carolina Biological Supply Company
9
© 2016 Carolina Biological Supply Company
Exploring Photosynthesis and Plant Pigments
Student Name
4/12/2022
Instructions:
1. Please read all of the introduction and background information within the
investigative manual.
a. Once you have done so answer the prelab question BEFORE
completing any of the lab’s activities.
2. Once you have completed the prelab questions proceed to the activities
of the lab within the investigative manual.
a. As you read through the instructions for completing each activity
make sure you also:
i. Complete any instructions (append photos, etc)/ and answer
any questions found in the post lab questions for each
activity.
ii. Take the photos of your experiments in each activity as
directed below. IMPORTANT: Don’t clean-up your lab until you
know what portion of the experiment you need to take a
picture of.
3. Here is a video that will introduce you to the lab and its main concepts.
The student is encouraged to watch it.
a. Exploring Photosynthesis and Plant Pigments
Prelab Questions
1. A typical chloroplast within a plant contains several pigments. Which are
the most common pigments and what color of light will they absorb?
Which color will they reflect?
Chlorophyll a and b, and carotenoids are the most common pigments
found in plants. The pigments absorb blue and red light, while reflecting all
the other colors (purple, yellow, green, and orange).
2. In consideration of the 1st question, why do tree leaves change color in
the autumn?
Every leaf’s chlorophyll loses light to produce pigmentation, and the colors
that are absorbed stay in the leaves. Green and purple pigments are
broken down by chlorophyll.
1
© 2016 Carolina Biological Supply Company
3. What two molecules are made in the light-dependent reactions of
photosynthesis? Are these “energy-containing” molecules? Where did
they get their energy from and how are these used in the Calvin cycle?
ATP and NADPH are the molecules that are made. ADP and NADP+ are
responsible for generating the energy in these molecules. These two
molecules convert carbon dioxide into sugar.
4. As stated in the investigative manual, in this lab we will be investigating
the effect of both light and concentration of CO2 on the rate of
photosynthesis. BEFORE you begin activity #2 please read through the
activity and fill out the purpose and hypothesis statements found below
under the activity #2 heading.
Activity 1
Instructions:
1. Open the investigative manual. Locate all the needed materials
supplied in the kit and those you will need to supply yourself.
2. Lay them out in your work area.
3. Read through the entire set of instructions found in the investigative
manual for the activity to avoid making mistakes when you go to
execute the experiment.
4. Once you have read through the instructions go back to step 1 and
begin executing the experiment.
5. Please answer the questions below and/or append appropriate
representations of data (photos, graphs, etc). REMEMBER don’t clean
up until you have taken the appropriate photos of your experiment as
described below.
Photo 1 – Activity 1
Take a picture and insert the image(s) of your chromatography paper from step
12. of the “Procedure” section in activity 1 of the investigative manual:
2
© 2016 Carolina Biological Supply Company
Data Table 1: Chromatography of Plant Pigments
Band #
Distance from origin (mm)
Band Color/ Identification
1
1.5
Light yellow
2
2.5
Clear
3
4.5
Green
4
5
Light yellow
Solvent
front (from
Step 14.)
5
1. Many things can affect the evaporation of solvents during a
chromatography experiment. To allow researchers to compare results across
experiments under different conditions, scientists use an Rf value, that is the
ratio of the distance the pigment travelled to the distance the solvent
travelled as shown in the equation below:
Rf = (distance band traveled/distance solvent from traveled)
This number remains constant across trials. Calculate the Rf value for each
pigment.
1. 0.3cm
2. 0.5cm
3. 0.9cm
4. 1cm
3
© 2016 Carolina Biological Supply Company
2. What do the calculated Rf values indicate about the pigment within that
particular band? In other words, did that pigment have more or less
interaction (compared to the other pigments) with the chromatography
paper during the experiment?
4
© 2016 Carolina Biological Supply Company
Activity 2
Instructions:
1. Open the investigative manual. Locate all the needed materials
supplied in the kit and those you will need to supply yourself.
2. Lay them out in your work area.
3. Read through the entire set of instructions found in the investigative
manual for the activity to avoid making mistakes when you go to
execute the experiment.
4. Once you have read through the instructions go back to step 1 and
begin executing the experiment. REMEMBER don’t clean up until you
have taken the appropriate photos of your experiment as described
below.
Purpose statement: (This should be the question the experiment is attempting to
address. It should be written as a question.)
When leaves are under different light conditions or CO2 concentrations, will their
rate of photosynthesis increase?
Hypothesis statement: (This should be an “if/then” testable prediction that
addresses the question/purpose of the lab.)
In conditions where the light level is high and the CO2 level is low, then there will
be more photosynthetic activity.
Evidence/Claim statement: (This should be a statement regarding whether your
hypothesis was supported or refuted and what data/evidence allows you to
make this claim.)
I have been proven right with my hypothesis. The experiment showed that more
disks floated when put into the light than when put into the dark.
Reflection statement: (This should be a statement of what you learned, how your
understanding changed, if you have new questions, and what connections can
you make between the lab and the content in the book and other
assignments.)
5
© 2016 Carolina Biological Supply Company
Before doing this experiment, I had no idea how different the settings of light
and air made a difference in photosynthesis. Because baking soda produces
carbon dioxide, it made it hard for the disks to float.
Photo 1 – Activity 1
Take a picture and insert the image(s) of your 0.12% syringe from step 16. of the
“Light Experiment” section in activity 2 of the investigative manual:
Data Table 2: Leaf Disk Assay, Light Treatment
Time
(minutes)
A
(0.24%)
B
(0.24%)
Average of
A and B
A
(0.12%)
B
(0.12%)
0
5
10
15
20
25
30
35
40
45
50
55
60
6
© 2016 Carolina Biological Supply Company
Average of
A and B
Data Table 3: Leaf Disk Assay, Dark Treatment
Time
(minutes)
A
(0.24%)
B
(0.24%)
Average of
A and B
A
(0.12%)
B
(0.12%)
Average of
A and B
0
5
10
15
20
25
30
35
40
45
50
55
60
Graph 1 – Activity 1
Make a single line graph of your results from both the light and dark
experiments. Your y-axis should be the average number of disks floating from A
and B (column four from your tables) and the x-axis should be time in minutes.
Insert your graph below:
7
© 2016 Carolina Biological Supply Company
1. Having now completed activity 2 and your tables go back and fill in your
Evidence/Claim and Reflection statements and then answer the question(s)
below.
2. For both the dark and the light trials identify the independent, the
dependent, and the control variables.
Independent Variable: is light and dark
Dependent variable: is the disk of the leaf
Control variable: is the amount of time in minutes
3. In this activity how do floating disks correspond to the rate of photosynthesis?
4. How did the baking soda solution affect photosynthetic rates? If you were to
conduct an experiment with a 0% baking soda solution would a
photosynthetic rate be observed? Why or why not?
5. Proceed now to the next assignment in D2L entitled “Lab Kit: Exploring
Photosynthesis and Plant Pigments Formal Report”. Instructions for writing the
formal report are found in D2L. Please submit the formal report as a separate
assignment from this one within the appropriate submission folder.
8
© 2016 Carolina Biological Supply Company
9
© 2016 Carolina Biological Supply Company
BIO111C46 Gen College Biology I/Lab: SC1 (Breean…
Module 3: Lab Kit: Exploring Photosynthesis and
Plant Pigments Formal Report Instructions
Module 3: Lab Kit: Exploring Photosynthesis
and Plant Pigments Formal Report
Instructions
Overview
This portion of the Exploring Photosynthesis and Plant pigments lab is the formal lab report.
The purpose is for you to briefly summarize activity two of the lab in the format of a formal
lab report to demonstrate an understanding of what you learned during the experimental
processes you conducted.
Instructions
1. Perform “Lab Kit: Exploring Photosynthesis and Plant Pigments” lab.
2. Conduct all lab exercises associated with this lab.
3. Prepare a report FOR ACTIVITY TWO (the formal report should not include Activity
One) that includes the following:
Abstract (A summary of the entire lab including purpose, hypothesis, results, and
conclusions)
Background and Introduction (Include background information on
photosynthesis, the purpose of the lab, the hypothesis for the lab)
Materials and Methods
Results (Worksheets and graph(s))
Discussion
Conclusions (Explain what you learned and whether your hypothesis correctly
predicted what you would learn)
Any possible references in APA format
4. Formal Lab Assignment Submission Instructions: Submit the completed Formal
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