Question 20 (4 points)5′ -GACCUUAUGCCCGAGUUACUAUAGGGCGU- 3′
In the image of an RNA transcript above, find the ORF. Write the complete sequence of the ORF,
and separate the codons with a single space: 5′-
A -3′
Question 21 (2 points)
Translate your ORF from the previous question using the three-letter amino acid abbreviations.
Separate each amino acid abbreviation with a single space.For this duplex of DNA, write the complementary mRNA transcript for the TOP
strand in a 5′ to 3′ orientation:
5′
3′.
3′ -TATCCGTACCTCCTTTTGGACCGGATTCC-
5′
5′ -ATAGGCATGGAGGAAAACCTGGCCTAAGG- 3′
(DO NOT include spaces between nucleotide ‘letters’.)
Question 34 (2 points)
3′
A/
5′.
For this duplex of DNA, COPY the BOTTOM DNA strand in a 3′ to 5′ orientation (left
to right):
3′ -TATCCGTACCTCCTTTTGGACCGGATTCC- 5′
5′ -ATAGGCATGGAGGAAAACCTGGCCTAAGG- 3′
A/(DO NOT include spaces between nucleotide ‘letters’.)
(Remember, you are only asked to COPY the DNA so that it easier to read in a left to
right, 3′ to 5′ direction.)
Question 35 (1 point)
For this duplex of DNA, write the complementary mRNA transcript for the BOTTOM
strand in a 5′ to 3′ orientation:
5′
3′
-TATCCGTACCTCCTTTTGGACCGGATTCC- 5′
5′ -ATAGGCATGGAGGAAAACCTGGCCTAAGG- 3′
3′.
A/
(DO NOT include spaces between nucleotide ‘letters’.
(Hint: Use your copied DNA strand in the above Question 34 to assist your efforts
for this question!)Question 37 (2 points)
Transcribe the nucleotide sequence of the ORF that you identified in the previous question.
Please separate the codons with a single space: 5′-
-3′
A
(Hint: You can use the answers to the previous questions to assist your efforts for this question!)
Question 38 (2 points)
Translate this ORF using the 3-letter amino acid abbreviations. Place a single space between the
abbreviations. Do not include “STOP”.
ABioinformatic Software
Analyze the entire sequence listed below this question with the ORF Finder and
BLAST software programs within NCBI.
Remember, within the ORF Finder software, under the ‘BLAST database’ option,
choose the “Non-redundant protein sequences (nr)” option and then select the
“BLAST” button (NOT the SmartBLAST).
CGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTATGTCTGA
TAATGGACCCCAAAATCACGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGA
TTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGT
CGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACAT
GGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAG
CAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTG
GTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCTACTACCTAGGAACTG
GGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCA
ACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAA
CAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTA
CGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTC
GCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGA
ATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAAC
CAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCAC
TAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTA
AAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGA
AATTTTGGGGACCAGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCA
AATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCAT
GGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATG
ACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCAT
ACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGA
AACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTG
CTGCAGATTTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACT
CAACTCAGGCCTAAATGGGGATAGCACTACTAAAATTAATTTTACACATTAGGGCTC
TTCCATATAGGCAGCTCTCCCTAGCATT
Question 39 (1 point)
For this question and the remaining questions, analyze the largest ORF in your results.
What is the reading frame of this ORF?
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